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M94A3176.TXT
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1994-10-25
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Document 3176
DOCN M94A3176
TI Characterization of enhancer-binding proteins that recognize HTLV-I LTR.
DT 9412
AU Nyunoya H; Shimotohno K; National Cancer Center Research Institute,
Tokyo, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):136 (abstract no. PA0165). Unique
Identifier : AIDSLINE ICA10/94369399
AB OBJECTIVE: The promoter activity of HTLV-I can be controlled by tax-gene
product as well as cyclic AMP or TPA. Factors related to the cellular
signal-transduction pathways may be involved in the transcriptional
control. To identify such factors, we have cloned cDNAs for multiple
enhancer-binding proteins and characterized them. METHODS:
Oligonucleotide probe containing the enhancer sequence was used for cDNA
cloning. DNA-binding specificity and other properties were examined by
using recombinant proteins made in E. coli. RESULTS: We have identified
three Tax-responsive element-binding proteins (TAXREB), which all have a
bZIP structure. TAXREB 67 and TAXREB302 recognized a core sequence
(TGACG) of cyclic AMP responsive element; TAXREB107 recognized the
downstream flanking sequence (TCCCCC). TAXREB67 was shown to be a
phosphoprotein in vivo and to be phosphorylated by cdc-2 kinase in
vitro. DISCUSSION AND CONCLUSIONS: The virus gene expression could be
regulated by multiple transcription factors bound to the LTR. Responses
to various extracellular stimuli may be different depending on
cell-type. Systematic analyses of such factors should be required for
further study.
DE Base Sequence Cloning, Molecular Comparative Study Cyclic
AMP/METABOLISM DNA-Binding Proteins/*METABOLISM DNA, Viral/*METABOLISM
*Enhancer Elements (Genetics) Escherichia coli Gene Expression
Regulation, Viral HTLV-I/*GENETICS/METABOLISM Oligonucleotide Probes
Promoter Regions (Genetics) Recombinant Proteins/METABOLISM
*Repetitive Sequences, Nucleic Acid Signal Transduction MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).